View the profiles of professionals named "Nathan Ellis" on LinkedIn. Selected genetic variants were genotyped in a discovery set of 27 MSS HNPCC cases and 85 controls. Lynn, H., Sun, X., Ayshiev, D., Siegler, J. H., Rizzo, A. N., Karnes, J. H., Gonzales Garay, M., Wang, T., Casanova, N., Camp, S. M., Ellis, N. A., & Garcia, J. G. (2018). Jacobs, E. T., Lance, P., Mandarino, L. J., Ellis, N. A., Chow, H. S., Foote, J., Martinez, J. Nathan Ellis' maiden ODI wicket is a beauty, and ends Imam-ul-Haq's innings on 103 Pakistan need 104 runs from the last ten overs, with four wickets in hand #PAKvAUS. Fanconi anaemia (FA), ataxia telangiectasia (A-T), Nijmegen breakage syndrome (NBS) and Bloom syndrome (BS) are clinically distinct, chromosome instability (or breakage) disorders. Rather, using DNA fiber assays, we found that the firing of new DNA replication origins, which is required for replication restart following prolonged stress, was inhibited in cells depleted of RNF4. To some of his friends he went by Nathan and with others, Dakota. Dr. Ellis obtained his PhD at the University of Washington studying X-chromosome inactivation and did post-doctoral training at the Imperial Cancer Research Fund analyzing the sex determining region of the Y chromosome. Nathan Ellis Director at Longcroft building services Bristol. Nathan Ellis - People Directory - 192.com We have found 165 people in the UK with the name Nathan Ellis. In the U.S., African Americans have higher rates of incidence and mortality in colorectal cancer. Because vitamin D is thought to protect against CRC and AAs have lower serum vitamin D levels, genetic variants that modulate the levels of active hormone in the tissues could explain some of the cancer health disparity. Loss-of-function mutations of , which codes for a RecQ helicase, cause Bloom's syndrome. Kohler, L. N., Foote, J., Kelley, C. P., Florea, A., Shelly, C., Chow, H. S., Hsu, P., Batai, K., Ellis, N., Saboda, K., Lance, P., & Jacobs, E. T. (2018). ", Carcinogenesis, vol. Nature 337:81 (1989) http://www.ncbi.nlm.nih.gov/pubmed/2594087, 2. Somatic intragenic recombination in Blooms syndrome provided an elegant method for molecular cloning BLM. It remains unclear whether these differences are the result of uncontrolled confounding in the observational studies, or whether there is a modest effect of Se on the risk for T2D that may vary by duration of exposure. PMID: 32293075, Pond, K. W., C. de Renty, M. K. Yagle, and N. A. Ellis, "Rescue of collapsed replication forks is dependent on NSMCE2 to prevent mitotic DNA damage. The BLM helicase has pivotal functions at the crossroads of DNA replication, recombination, and repair. Workin' on the Hill Nathan Ellis - @mambwlski Ynysybwl result was based on 1,244 Ashkenazi Jewish colorectal cancer cases and, with more than a two fold excess frequency of blmAsh in cases compared to controls observed, the result will be difficult to overturn without very large series (on the order to >15,000 cases). In 1990, he joined the New York Blood Center where, with James German and Joanna Groden, he characterized the molecular genetics of the gene mutated in Blooms syndrome BLM. Get the latest business insights from Dun & Bradstreet. This study provides a comprehensive eQTL map of distal colonic samples obtained from 40 healthy African Americans and demonstrates their relevance for GWAS of colonic diseases. Internationally, Ellis made headlines as the first Australian to take a hat trick on his T20 debut in the recent series against Bangladesh, with his performances earning him a callup to the 2021 T20 World Cup Squad as injury cover. They are part of Marketing Department and their management level is Manager. By comparing Ashkenazi Jewish cancer cases and controls, Ellis with Steve Gruber, Gad Rennert, and Ken Offit found that BLM+/- heterozygotes have approximately two times the risk of developing colorectal cancer (CRC) compared to BLM+/+ (14). (2016). By nationality, he is Australian and currently, his food habit is non-vegetarian. When Nathan Veatch Ellis was born on 10 November 1749, in Wales, United Kingdom, his father, James Ellis Sr., was 26 and his mother, Mary Hepburn Veatch, was 21. Ashktorab, H., Ahuja, S., Kannan, L., Llor, X., Ellis, N. A., Xicola, R. M., Laiyemo, A. O., Carethers, J. M., Brim, H., & Nouraie, M. (2016). Decreased copy-neutral loss of heterozygosity in African American colorectal cancers. The groups can be races, age groups, economic strata, and so on. In order to do so, coding regions in five candidate genes (MGMT, AXIN2, CTNNB1, TGFBR1 and TGFBR2) were sequenced in 11 unrelated microsatellite-stable hereditary non-polyposis CRC (MSS HNPCC) cases. Find out more. The MYLKP1 promoter is minimally active in normal bronchial epithelial cells but highly active in lung adenocarcinoma cells. Film, TV & Theatre. Selenium supplementation and insulin resistance in a randomized, clinical trial. More recently, aberrant transcriptional regulation of growth and survival genes has been proposed as a hypothesis to explain features of BS. MR Nathan Mark Ellis works at LONGCROFT BUILDING SERVICES LIMITED as Director, KINGSWOOD JOINERY LTD as Director and worked at DAY & ELLIS LTD as Director . She was sworn to her application (DAR File 137691) 26 Sep 1917, and also stated that Nathan Ellis was born in Wales. He was particularly fortunate during this time to collaborate with Ken Offit, who ran the Clinical Genetics Services in the erstwhile Department of Human Genetics. In the present study, we used publicly available data from The Cancer Genome Atlas (TCGA) as well as data from an African American CRC cohort, the Chicago Colorectal Cancer Consortium (CCCC), to compare frequencies of CNVs and cnLOH events in CRCs in the two racial groups. Two of the 45 tumors contained POLE mutations. The intestinal microbiota is postulated to modulate the effects of diet in promoting or preventing CRC. With these genetic aspects of Blooms syndrome clarified, the time seemed right to tackle one of the most mysterious aspects of Blooms syndromes genetics: In Blooms syndrome, about one third of patients exhibit somatic mosaicism, that is, the presence of functionally normal, low-SCE cells and mutant, high-SCE cells in blood lymphocytes of the same patient. More specifically, in states along the US-Mexico border, HAs and NAs have higher kidney cancer incidence rates as well as a higher prevalence of kidney cancer risk factors, including obesity and chronic kidney disease. Cell 63: 977 (1990) http://www.ncbi.nlm.nih.gov/pubmed/2124175, 4. The BLM mutation that is common in the Ashkenazi Jewish population, which we refer to as blmAsh, is a unique Blooms syndrome-causing mutation specific to Ashkenazi Jews; consequently, a straightforward PCR assay could be devised to test whether BLM+/- heterozygotes are at increased risk of cancer. We conclude that some CRC risk alleles are shared between EAs and AAs and others are population specific. BMC Med Genet 4:1 (2003) http://www.ncbi.nlm.nih.gov/pubmed/12529183, 18. Nathan Ellis; Related To: Darrel Ellis; Willie Farley, 59; David Wray, 70; James Ellis, 79; Timothy Ellis; Phones (972) 203-4402 (903) 628-8344 (469) 726-0367 (972) 726-0367 . The excessive homologous recombination in Blooms syndroem is mutagenic when the recombination occurs between homologous chromosomes. Further investigations on the effects of Se on glucose metabolism are needed. In 2020 his play SUPER HIGH RESOLUTION was Shortlisted for the Verity Bargate Award. (Employees figure is estimated, Sales figure is modelled). Genomic instability is a dominant feature of cancer cells, and it is the central phenotype of a large group of clinical entities in which cancer development is the main and often fatal consequence. The primary purpose of this work was to determine the relationship between plasma levels of oxylipins and colorectal adenoma characteristics at study entry, as well as with the development of a new adenoma during follow-up within a Phase III adenoma prevention clinical trial with selenium (Sel). Both of these mechanisms have the effect of correcting the mutated BLM gene to generate a functionally normal BLM gene. Ellis and German also showed that there is at least one other molecular mechanism in Blooms syndrome (back mutation) that can explain the somatic mosaicism (10). Film, TV & Theatre . Sold: 2 beds, 1 bath, 720 sq. In the combined genotype data, rs67687202 was associated with a moderate increase in CRC risk (OR = 1.68; 95% CI = 1.13-2.50; P = 0.010). (2016). (2014). Tel: 520-626-1197 | Fax: 520-626-1460 | Admin Login Replication delay and slower cell turnover rates have been proposed to explain many features of BS, such as short stature. Lakeside . Everton transfer news, rumours, gossip and speculation including updates on Kamaldeen Sulemana, Anthony Elanga, Danny Ings, Duvan Zapata, plus manager Frank Lampard and owner Farhad Moshiri Thus, rs868 potentially is a CRC risk-causing allele. Instead, these Lynch-like syndrome (LLS) patients have somatic mutations in MMR genes. 290 genes were differentially expressed between tumors with high and low stage, size, grade, and necrosis (SSIGN) score (7 . Shaag et al. We are investigating mechanisms leading to the excess right-sided colon cancer and early-onset colorectal cancer in the African American population. Gasteroenterology 139:1677 (2010) http://www.ncbi.nlm.nih.gov/pubmed/20659471, 26. He made his international debut for the Australia cricket team in August 2021. In that application she stated that Nathan Ellis was born in Wales. The term race refers to groups of people that are thought by the socially dominant group as similar (appearance, geographic location of origins, language, and so on). Nathan Ellis Profile Ellis is a right-arm medium-fast bowler from Australia and is known for his impressive death bowling skills in the Big Bash League (BBL). They present with more right-sided, microsatellite stable disease and are diagnosed at earlier ages compared with non-Hispanic Whites (NHWs). His play work.txt (a play without actors) ( Observer) and was Offie-Nominated in 2022. Six hundred and fifty-four consecutive CRC patients were screened for suspected LS using MSI and absence of MLH1 methylation. Gold et al. Mol Biol Cell 10:665 (1999) http://www.ncbi.nlm.nih.gov/pubmed/10069810, 12. DICE Looks to reduce and eliminate barriers . This unusual observation had been unexplained. NATHAN ELLIS YACHTING LIMITED has 2 employees at this location and generates $104,613 in sales (USD). Ellis has been using genetics to compare genetic factors in different ethnic groups to investigate colorectal cancer susceptibility genes. rs868 is contained in a let-7 miRNA binding site in the 3'UTR of TGFBR1, which might provide a functional basis for the association in MSS HNPCC. In this study, we further validate MYLKP1 as an oncogene via elucidation of the functional role of MYLKP1 genetic variants in colon cancer risk. A diet high in animal protein and fat is an environmental risk factor for CRC development. Nathan Ellis in South Carolina. Tippett and Ellis, Transfusion Med Rev 12:233 (1998) http://www.ncbi.nlm.nih.gov/pubmed/9798268, 7. Nathan Ellis height is --. When BLM was inherited in a person identical-by-descent from a common ancestor, the occurrence of moscaicism was rare. Grant, A., Xicola, R. M., Nguyen, V., Lim, J., Thorne, C., Salhia, B., Llor, X., Ellis, N., & Padi, M. (2021). Indeed, since 2000, during which time colonoscopy became widely used as a screening tool, the overall incidence of CRC has been reduced by 29%. These trainees have come from a wide variety of ethnic backgrounds, including persons of Hispanic and Native American ancestry. PMCID: PMC6292413 PMID: 30239619, Augustus, G. Julian, D. J. Roe, E. T. Jacobs, P. Lance, and N. A. Ellis, "Is increased colorectal screening effective in preventing distant disease? Mechanisms underlying this observation have not been proposed, nor is it clear whether an association between Thr300Ala and clinical outcomes will be observed in other cancers. The Weight can be changed regularly, here we have added the latest value. Nathan Ellis Owner at Ellis Electric Inc. Lexington, SC. From the standpoint of genetic epidemiology, the variation in ancestry is a possible source of confounding, for example, if cases and controls draw on persons with different proportions of genetic ancestries. An introduction to homologous recombination and replication fork stability. MLS# 22203743. Although some of these disparities can be explained by differences in access to care, cancer screening, and other socioeconomic factors, disparities remain after adjustment for these factors. Ellis, who has three international caps, joins compatriot Ben McDermott in the Hampshire ranks for the 2022 tournament. Nathan Mitchell advises clients on national security matters, including reviews by the Committee on Foreign Investment in the United States (CFIUS), the Defense Counterintelligence and Security Agency (DCSA), and Team Telecom, as well as on cybersecurity and . Nathan Ellis, PhD Director, Division of Cancer Genomics, Center for Applied Genetics and Genomic Medicine Scientific Director, Cancer Biology Research Program Associate Professor, Cellular and Molecular Medicine Most recently, the Ellis laboratory has pivoted to the analysis of the cancer genome to better understand the role of genetic and environmental factors in cancer health disparities. (A) Quantitative analysis of G1 arrest after release of control and NSMCE2-depleted HeLa cells from HU block into normal medium . Sports Fan. The goals of this project are to understand the molecular and genetic mechanisms that mediate risk of colorectal cancer and to examine mechanisms of carcinogenesis. BIG BASH CAREER STATS OVERALL BBL|12 BBL|11 BBL|10 BBL|09 A Germline Variant on Chromosome 4q31.1 Associates with Susceptibility to Developing Colon Cancer Metastasis. Ellis, N., Zhu, J., Yagle, M. K., Yang, W. C., Huang, J., Kwako, A., Seidman, M. M., & Matunis, M. J. Chicago native Nathan Ellis brings an energy to events, infusing a diverse background and story telling skills to provide a unique experience. Most LLS tumors had a combination of somatic MMR gene mutation and loss of heterozygosity. Nathan Ellis Chief Product Designer at Michael Design Cincinnati, OH. Nathan Rhys Ellis Director See All Contacts Dynamic search and list-building capabilities Real-time trigger alerts Comprehensive company profiles Valuable research and technology reports Get a D&B Hoovers Free Trial Financial Data Dun & Bradstreet collects private company financials for more than 23 million companies worldwide. Selenium supplementation was associated with a significantly smaller increase in 5-HETE after 12 months compared to the placebo, though no other results were statistically significant. Butlin's Big Weekenders, exclusively for adults, has announced a brand-new break, We Call It Dance. 4919 Tucson, AZ 85724 naellis@arizona.edu Bio Interests Courses Of the 13 observational studies included, 8 demonstrated a statistically significant positive association between concentrations of Se and odds for T2D, with odds ratios (95% confidence intervals) ranging from 1.52 (1.012.28) to 7.64 (3.3417.46), and a summary odds ratio (OR) (95% confidence interval (CI)) of 2.03 (1.512.72). [2] Career [ edit] Am J Hum Genet. WS is a prototypic premature aging disorder; however, the clinical features present in BS and RTS do not indicate accelerated aging. 1501 N. Campbell Avenue | PO Box 245017 | Tucson, Arizona 85724 Augustus, G. J., & Ellis, N. A. Nate is admitted to the State Bar of Montana and the U.S. District Court for the District of Montana. Many colorectal cancers (CRCs) that exhibit microsatellite instability (MSI) are not explained by MLH1 promoter methylation or germline mutations in mismatch repair (MMR) genes, which cause Lynch syndrome (LS). Carcinogenesis April 21 (2014) http://www.ncbi.nlm.nih.gov/pubmed/24753543, 27. He is developing TV projects with Balloon, Archery Pictures, UFA Fiction, and Tall Story Pictures, and is writing a feature for Calamity Films with Rene Zellweger attached. The double-strand break (DSB) is the most cytotoxic type of DNA damage and measurement of DSBs in cells is essential to understand their induction and repair. Nathan Snow Ellis III, a former Falmouth selectman, DPW director and county commissioner, died October 18 at Falmouth Hospital following a brief illness. LByrne@unitedagents.co.uk. A., Skiba, M. B., Chow, H. S., Chew, W. M., Saboda, K., Lance, P., Ellis, N. A., & Jacobs, E. T. (2021). Specialist clinics for each disorder have provided the concentration of expertise needed to tackle their characteristic clinical problems and improve outcomes. Minneapolis, Minnesota. . An important realization has been the predisposition to cancer in carriers of some of these gene mutations. Can Res 64: 8116 (2004) http://www.ncbi.nlm.nih.gov/pubmed/15520224, 22. Nathan Ellis #8 Pelham Panthers / GOJHL - 18/19 Premium Features Discover premium 3 553 Profile Analytics Enter Edit Mode Request to Update Facts & Stats Player Facts Date of Birth May 29, 1998 Position D Age 24 Height 6'0" / 183 cm Place of Birth Fonthill, ON, CAN Weight 181 lbs / 82 kg Nation Canada Shoots L Youth Team - Contract - However, the functional role of many of these SNPs is largely unknown and tissue-specific resources are lacking. Click here to find personal data about Nathan Ellis including phone numbers, addresses, directorships, electoral roll information, related property prices and other useful information. While many factors cause population structure (genetic aggregations) such as social status, religious and moral values, shared language, customs and so on, if the potential mate lived on another continent, the probability of a marriage was very small. The objective is to elucidate the basis of these cancer disparities. Gradually, he developed an obsession with fast-bowling. We identified 23 suspected LS cases. Blooms syndrome was known to be a Jewish genetic disease, because the syndrome, albeit rare, is relatively more frequent is persons whose parents are Ashkenazi Jewish than in other human populations. LD is defined as the excess co-occurrence of two alleles over that which is expected at random. Colorectal Cancer Disparity in African Americans: Risk Factors and Carcinogenic Mechanisms. Nathan Ellis Audit Associate at Grant Thornton International Ltd Accounting . These DNA methylation findings suggest potential cellular mechanisms associated with increased cancer risk in firefighters. University of Arizona College of Medicine Tucson These results are raising afresh questions regarding the interplay of genetic ancestry and environmental influences in colorectal carcinogenesis. Nathan Ellis. Genome-wide methylation predicted with high accuracy incumbent and new recruit status as well as years of service among incumbent firefighters. Colorectal cancer in African Americans is more often right-sided and more often diagnosed at an earlier age compared to white populations (27). (2018). (2019). Nathan Ellis (I) Writer Director Producer IMDbPro Starmeter See rank Nathan Ellis is a former Army Captain, military aviator and highly decorated combat veteran who believes his experiences while in service have played an important role in his development as an artist. Shared and independent colorectal cancer risk alleles in TGF-related genes in African and European Americans. Ellis et al. Gene expression was determined using whole-transcriptome sequencing. In 1997, Ellis took a position at Memorial Sloan-Kettering Cancer Center, investigating the genetic epidemiology of colorectal cancer. Kupfer et al. (2014). This review reports that kidney and renal pelvis cancers are unevenly affecting HAs and NAs compared to European Americans of non-Hispanic origin, and that currently there is significant need for improved data and reporting to be able to advance toward genomic-based precision medicine for the assessment of such cancers in these medically underserved populations. Un-rescued forks persist into mitosis, leading to increased mitotic DNA damage. Gruber et al. B., Janoski, A., Pusatcioglu, C. K., Rajaram, P., Gluskin, A. Enrichment of inflammatory bowel disease and colorectal cancer risk variants in colon expression quantitative trait loci. Augustus, G. J., Xicola, R. M., Llor, X., & Ellis, N. A. Read More Nathan W Ellis , 37 Lives in Des Moines, IA Related to: William Eric Ellis, Theresa Kay Ellis, Erica R Ellis From the statistical perspective, estimation of the frequency of rare variants (minor allele frequency less than 1%) is plagued by power issues (15). Adaptation of Mahlers Second Symphony, about terrorism and mental health, directed by director Iqbal Khan (RSC). His first play NO ONE ISCOMING TO SAVE YOU ('a blazing debut' The Stage) was invited to Incoming Festival 2019 and toured nationally. Writer. University Privacy Statement In Ellis early years, he studied X chromosome inactivation with Stanley M. Gartler at the University of Washington and sex determination with Peter N. Goodfellow at the Imperial Cancer Research Fund. This observation suggested that, in order for a person with Blooms syndrome to exhibit the high-SCE/low-SCE mosaicism, that person had to be a genetic compound with disease-causing mutations at different positions within BLM. Hum Mol Genet 14:555 (2005) http://www.ncbi.nlm.nih.gov/pubmed/15649950, 21. Consequently, we hypothesized that genetic variants in vitamin D-related genes are associated with CRC risk. Cancer mortality, however, remains a persistent difference. On 01/09/2023 LEAKS, NATHAN ELLIS filed an Other - Other Criminal court case in Los Angeles County Superior Courts. He can vary his pace and bowl slower balls at will. Several studies have investigated the potential role of selenium (Se) in the development of type 2 diabetes (T2D) with disparate findings. Find company research, competitor information, contact details & financial data for NATHAN ELLIS LTD of COVENTRY. Study of the molecular and genetic basis of these disorders has revealed mechanisms of recognition and repair of DNA double-strand breaks, DNA interstrand crosslinks and DNA damage during DNA replication. I lead a project to identify and characterize the molecular cancer genetics of colon and rectum in African Americans, which has been supported by a grant from the NCI Center to Reduce Cancer Health Disparities (U01CA153060). NATHAN ELLIS APPAREL DESIGN / MERCHANDISING / BRAND CONCEPT / LICENSING. Firefighters are exposed to carcinogens and have elevated cancer rates. The possibility of hereditary factors in LLS warrants further studies so counseling can be properly informed. rs60745952, located in an intergenic region on chromosome 4q31.1, and not previously associated with cancer, is, thus, a germline genetic marker for susceptibility to developing colon cancer metastases among Ashkenazi Jews. Sixteen studies from 15 papers met inclusion criteria defined for this review. In the method described here, molecules from five hundred thousand to three million base pairs are consolidated into a single band on the gel that is readily analyzed. Bloom's syndrome is an autosomal recessive disorder characterized by prenatal and postnatal growth deficiency, photosensitive skin changes, immune deficiency, insulin resistance, and a greatly increased risk of early onset of cancer and for the development of multiple cancers. To address this hypothesis, we collected peripheral blood samples from 45 incumbent and 41 new recruit non-smoking male firefighters and analyzed the samples for DNA methylation using an Illumina Methylation EPIC 850k chip. Nature 344:663 (1990) http://www.ncbi.nlm.nih.gov/pubmed/2325773, 3. The 27-year-old will add pace and energy to the Hawks' bowling line-up, and will give captain James Vince a reliable option with the ball at the death. The results will very likely lead only to better localization of those shared risk alleles but also to new genetic risk factors at play in CRC susceptibility. Nathan is a current member of the Channel 4 4Screenwriting group and was a member of BBC WritersRoom Drama Room 2021-22. Hereditary Colorectal Cancer. German noticed that, of the persons with Blooms syndrome who exhibited the high-SCE/low-SCE somatic mosaicism, very few had parents who were related as cousins or who were Ashkenazi Jewish. The Deaf & Immigrant Center for Education (DICE) is an educational support program utilizing uniquely experienced Community Health Workers throughout various stages of patient intervention and care. Nathan is a current member of the Channel 4 4Screenwriting group and was a member of BBC WritersRoom Drama Room 2021-22. . Dad. Recent studies have found that early-onset CRC exhibits an increased proportion of tumors lacking an APC mutation. Some investigators consider this result controversial because reports from several other groups of smaller series did not observe an effect of BLM+/- on cancer susceptibility. He serves as Program Director of the Cancer Biology Program in the University of Arizona Cancer Center and Director of the Genetics graduate program. Offit and Ellis initially worked together to examine the role of heterozygosity of caretaker genes like BLM and ATM in conferring risk to cancer. 1983-1994, 2017 11. It was performed at the Soho Theatre in 2022 directed by Blanche McIntyre ( Daily Mail) and will enter the repertoire Staatstheater Kassel in 2023 directed by Manon Pfrunder. In his free time Nate enjoys camping with his family, riding and . Pond, K. W., & Ellis, N. A. Nathan Ellis Associate Professor, Cellular and Molecular Medicine Chair, Genetics - GIDP Associate Professor, Cancer Biology - GIDP Co-Program Leader, Cancer Biology Research Program Member of the Graduate Faculty Contact (520) 626-7979 Leon Levy Cancer Center, Rm. Nathan Ellis - @NathanEllis_SC Columbia, SC South Carolina Football Recruiting Assistant Nathan Ellis-Calcott - @NathanCalcott propcapltd.co.uk PropCap Consultancy Ltd info@propcapltd.co.uk Nathan Ellis - @NathanEJEllis Ottawa, Ontario Husband. Overall BBL|12 BBL|11 BBL|10 BBL|09 a Germline Variant on Chromosome 4q31.1 Associates with susceptibility to Developing colon cancer and colorectal... Lls ) patients have somatic mutations in MMR genes which is expected at random M.... Was a member of BBC WritersRoom Drama Room 2021-22. ) http: //www.ncbi.nlm.nih.gov/pubmed/12529183, 18 & # x27 s... Biol cell 10:665 ( 1999 ) http: //www.ncbi.nlm.nih.gov/pubmed/2124175, 4 needed to their... 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